Genejet plasmid miniprep kit protocol pdf

# Genejet plasmid miniprep kit protocol pdf
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Each GeneJET spin column can recover up toµg of plasmid DNA. The kit can be successefully used for efficient purification of any size plasmids and cosmids Quick Protocol Note. Centrifuge empty column High quality plasmid DNA can be obtained from various strains including DH10B, DH5α, XL1-Blue, JM, JM, TOP Culture media The GeneJET Plasmid The GeneJET™ Plasmid Miniprep Kit is designed for rapid and cost-effective small-scale preparation of high quality plasmid DNA from recombinant cultures. The GeneJET Plasmid Miniprep Kit is designed for rapid and cost-effective small-scale preparation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column Harvest the bacterial culture by centrifugation at rpm (x g) in a microcentrifuge forminutes at room temperature The GeneJET Plasmid Midi Prep Kit provides optimized protocols for plasmid DNA purification using low speed (up to 5, × g) and high speed (up to, × g) centrifuges, as well as using vacuum manifolds The GeneJET™Plasmid Miniprep Kit is designed for rapid and cost-effective small-scale preparation of high quality plasmid DNA from recombinant cultures. All centrifugations should be carried out in a microcentrifuge at ≥x g (rpm, depending on the rotor type). GeneJET Plasmid Miniprep Kit K, K Wash the column Add μL of Wash Solution and centrifuge for s. Harvest bacteria. Each GeneJET™ spin column can recover up toµg of plasmid DNA Protocol. The The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. GeneJET Plasmid Miniprep Kit, K Introduction. The kit Plasmid miniprep Introduction This protocol describes the steps in the GeneJET plasmid miniprep kit, in which plasmids can be extracted and purified from cell samples. Forpreps. Ea ch GeneJET spin column can recover up toµg of plasmid DNApreparation of high quality plasmid DNA from recombinant E. coli cultures. The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. All steps should be carried out at room temperature. The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column. Discard the flow-through.