Puc19 vector pdf
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Description pUCis a commonly used, high copy number cloning vector. This linearized version was Product. Corpus IDpUCis a small, high-copy number E. coli plasmid cloning vector containing portions of pBR and M13mp(1). Description. Explore Over k Plasmids: Basic Cloning Vectors More Plasmid Sets The pUCLinearized Vector is provided as a part of In-Fusion® Cloning Kits, and is intended to serve as a control for In-Fusion cloning. Plasmids [PDF] Construction of an enlarged pUCvector with a rop gene designed to study plasmid maintenance in Escherichia coli Semantic Scholar. Description. It has been constructed using the ampicillin resistance gene and the pMB1 origin of replication from pBR pUCis a small, high copy cloning vector for pUCvector. Try SnapGene for Free. Download SnapGene Viewer. The MCS is reversed in pUC Sequence Author: New England Biolabs pUCLinearized Vector Map and Multiple Cloning Sites (MCS). Open in SnapGene. It con-tains the pMB1 origin of replication from pBR, but it lacks the rop gene and carries a point mutation in the RNAII transcript. Download Plasmid. Keywords,,,,,,,,,,,,,,,,,,,,,,, The small size of pUCvector (bp) allowed more detailed analysis of ligation efficiency as well as ligation patterns. The MCS is reversed in pUC Sequence Author: New England Biolabs. pUChigh copy cloning vector for replication in E. coli, suitable for “blue-white screening” technique. (G in pBR to A in pUC19; 2) pUC Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. Furthermore, in preparation for studying the effect of plasmid size on ligation efficiencies, cloning experiments were performed to generate three new pUCbased vectors Product. MATERIALS AND METHODS Plasmids. This mutant plasmid will be electroporated into E. coli DH5α and will be selected for, amplified and purified. pUChigh copy cloning vector for replication in E. coli, suitable for “blue-white screening” technique. pUCSequence and Map. Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. pUCis a small, high copy cloning vector for replication in E. coli.