Abts assay protocol pdf
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The ABTS radical scavenging activity was assessed using a method described by Yu et al. Download scientific diagram Experimental protocol of ABTS assay to assess the antioxidant activity of EOs. from publication: In vitro Methods to Study Antioxidant and 1, · Technical Manual. Add μL of ABTS Solution you have previously prepared (see Reagent preparation) in each well. Total Antioxidant Capacity (T-AOC) Colorimetric Assay Kit (ABTS, Chemical Method) Catalogue Code: MAES SizeT. Read the absorbance at nm at aboutoC Two antioxidant activity tests, including the analysis of the cation radical (ABTS) and the analysis of the reducing antioxidant capacity of the cupric ion (CUPRAC), as well as a test for the total phenolic content, the Folin–Ciocalteu test (FC), were used at the same time The ABTS solution was prepared by mixing an equal volume of ammol/L ABTS stock solution with a mmol/L potassium persulfate solution. Antioxidant compounds quench the color and produce a oloration of the solution which is proportional to their amount This protocol describes how to perform the ABTS olorization assay to assess potential in vitro antioxidant capacity of molecules and extracts using microtiter plates Download scientific diagram Experimental protocol of ABTS assay to assess the antioxidant activity of EOs. from publication: In vitro Methods to Study Antioxidant and Some Biological Short protocol: Prepare all reagents andwell plate. with modifications. Research Use ABTS Assay. The mixture was then stored in the dark at room temperature for In our assay a solution of ABTS at neutral pH and in the presence of a suitable solution, can form a stable and colored radical cation (ABTS•+) which shows a maximum of absorbance at nm. It is based on the principle that when ABTS (2,2’-azino-bis(3-ethylbenz-thiazolinesul-fonic acid) is incubated with a proper chemical, an , · ABTS assay. Mix the mixture forminutes under continuous stirring. AddμL of the sample standard in each well. In the ABTS assay, also known as Trolox equivalent antioxidant capacity (TEAC) assay, the green–blue stable radical cationic chromophore, 2,2-azinobis-( TEAC assay Trolox equivalent antioxidant capacity (TEAC) assay is generally based on the ability of antioxidants presenting in a sample in reduce or inhibit oxidized products generated in the assay.